Acta Anatomica Sinica

›› 2009, Vol. 40 ›› Issue (2): 340-343.doi: 10.3969/j.issn.0529-1356.2009.02.035

• 技术方法 • Previous Articles    

Selection of developmentally competent pig oocytes using brilliant cresyl blue staining

  

  1. 1.College of Life Science, Northeast Agricultural University, Haerbin 150030, China;2.Department of Materials Science and Engineering, College of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China
  • Received:2007-12-19 Revised:2008-04-08 Online:2009-04-06
  • Contact: LIU Zhong-hua

Abstract: P>Objective To enhance efficiency of EM>in vitro/EM> embryos culture system of pig using brilliant cresyl blue (BCB) stain. Methods To stain the cumulusoocyte complexes through BCB then evaluate oocytes maturation rates and developmental competence of early embryos. Results The percentage (91.6%) of the MⅡ stage oocytes of pigs was higher in the BCBcolorable group (BCBSUP>+/SUP> ) than those (64.0%) in the noncolorable (BCBSUP>－/SUP>) and control (77.5%) groups (EM>P/EM><0.05). The pig parthenogenetic blastocyst rate (34.9%) was significantly higher in the BCB+ group than those in the control and BCBSUP>－/SUP> groups, (23% and 9.5%) respectively (EM>P/EM><0.05). The rate (23.9%) of pig somatic cell nucleus-transferred embryos into blastocyst was significantly higher in the BCBSUP>+/SUP> group than those in the control and the BCB

Key words: Oocyte, Nuclear transfer, Brilliant cresyl blue （BCB）staining, Pig

CLC Number: